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細(xì)胞中文名稱人髓母細(xì)胞瘤細(xì)胞
細(xì)胞英文名稱Daoy
物種人
細(xì)胞形態(tài)特征多角
細(xì)胞生長特性人髓母細(xì)胞瘤細(xì)胞Daoy細(xì)胞 貼壁生長
細(xì)胞特種特性 該細(xì)胞是1985年由Jacobsen PF建立的,源自一名4歲大的兒童的后顱窩腫瘤的活檢組織,未檢出神經(jīng)元和膠質(zhì)分化的特征;可用作轉(zhuǎn)染宿主
培養(yǎng)基DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose)
血清10%FBS
細(xì)胞傳代方法1:4~1:6傳代;每周換液2~3次
細(xì)胞傳代情況C4
細(xì)胞凍存條件基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
支原體檢測陰性
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