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細(xì)胞中文名稱大鼠骨骼肌成肌細(xì)胞
細(xì)胞英文名稱L6
物種大鼠
細(xì)胞形態(tài)特征成肌細(xì)胞樣
細(xì)胞生長(zhǎng)特性大鼠骨骼肌成肌細(xì)胞L6細(xì)胞貼壁生長(zhǎng)
細(xì)胞特種特性 該細(xì)胞是Yaffe在甲基膽蒽存在的情況下從大鼠大腿肌原代培養(yǎng)的zui初兩代細(xì)胞中分離得到的;在培養(yǎng)基中融合形成多核的肌管和橫紋肌纖維,細(xì)胞融合的程度隨著代數(shù)的增加而下降,因此細(xì)胞應(yīng)低代次冷凍并周期性地重新克隆以選擇融合能力強(qiáng)的細(xì)胞。鼠痘病毒陰性。該細(xì)胞應(yīng)在達(dá)匯合狀態(tài)前傳代,以延緩細(xì)胞分化能力的喪失
培養(yǎng)基DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose)
血清10%FBS
細(xì)胞傳代方法1:20~1:40傳代;每周換液2~3次
細(xì)胞傳代情況C3
細(xì)胞凍存條件基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
支原體檢測(cè)陰性
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