本產(chǎn)品僅用于科研，不用于臨床

英文名稱 Anti-phospho-c-Raf（Ser339）
中文名稱 磷酸化原癌基因c-Raf抗體
別 名 RAF1（phospho S339）; RAF1; murine leukemia viral （v-raf-1） oncogene homolog 1 （3611-MSV）; v-raf-1 murine leukemia viral oncogene homolog 1; cRaf; C-RAF; proto-oncogene c-RAF; 6430402F14Rik; AA990557; BB129353; c-Raf; Craf1; D830050J10Rik; MGC102375; Raf-1; Raf 1; v-Raf; c Raf; Craf 1 transforming gene; Craf1 transforming gene; EC 2.7.11.1; Murine sarcoma 3611 oncogene 1; RAF; Raf proto oncogene serine/threonine protein kinase; vraf1 murine leukemia viral oncogene homolog 1; c-Raf.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep
產(chǎn)品類型 一抗 磷酸化抗體
研究領(lǐng)域 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 細胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 73kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human c-Raf around the phosphorylation site of Ser339
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The Raf family of serine/threonine specific kinases is comprised of three members （aRaf, bRaf, and cRaf） that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase （MAPK） pathway. cRaf kinase （also known as Raf1） is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.
Function : Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase （MAPK） cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases （MAP2K1/MEK1 and MAP2K2/MEK2） and the extracellular signal-regulated kinases （MAPK3/ERK1 and MAPK1/ERK2）. The phosphorylated form of RAF1 （on residues Ser-338 and Ser-339, by PAK1） phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility （ROCK2）, apoptosis （MAP3K5/ASK1 and STK3/MST2）, proliferation and angiogenesis （RB1）. Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin （OCLN） by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. [CATALYTIC ACTIVITY] ATP + a protein = ADP + a phosphoprotein.
Subunit : Monomer. Homodimer. Heterodimerizes with BRAF and this heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer. Forms a multiprotein complex with Ras （M-Ras/MRAS）, SHOC2 and protein phosphatase 1 （PPP1CA, PPP1CB and PPP1CC）. Interacts with Ras proteins; the interaction is antagonized by RIN1. Weakly interacts with RIT1. Interacts （via N-terminus） with RGS14 （via RBD domains）; the interaction mediates the formation of a ternary complex with BRAF, a ternary complex inhibited by GNAI1 （By similarity）. Interacts with STK3/MST2; the interaction inhibits its pro-apoptotic activity. Interacts （when phosphorylated at Ser-259） with YWHAZ （unphosphorylated at 'Thr-232'）. Interacts with MAP2K1/MEK1 and MAP2K2/MEK2 （By similarity）. Interacts with MAP3K5/ASF1 （via N-terminus） and this interaction inhibits the proapoptotic function of MAP3K5/ASK1. Interacts with PAK1 （via kinase domain）. The phosphorylated form interacts with PIN1. The Ser-338 and Ser-339 phosphorylated form （by PAK1） interacts with BCL2. Interacts with PEBP1/RKIP and this interaction is enhanced if RAF1 is phosphorylated on residues Ser-338, Ser-339, Tyr-340 and Tyr-341. Interacts with ADCY2, ADCY5, ADCY6, DGKH, RCAN1/DSCR1, ROCK2, PPP1R12A, PKB/AKT1, PPP2CA, PPP2R1B, SPRY2, SPRY4, CNKSR1/CNK1, KSR2 and PHB/prohibitin.
Subcellular Location : Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Note=Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.
Tissue Specificity : In skeletal muscle, isoform 1 is more abundant than isoform 2. [PTM] Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269, Ser-338, Tyr-341, Thr-491 and Ser-494 results in its activation. Phosphorylation at Ser-29, Ser-43, Ser-289, Ser-296, Ser-301 and Ser-642 by MAPK1/ERK2 results in its inactivation. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity. Phosphorylation at Ser-338 by PAK1 and PAK7/PAK5 and Ser-339 by PAK1 is required for its mitochondrial localization.
DISEASE : Defects in RAF1 are the cause of Noonan syndrome type 5 （NS5） [MIM:611553]. Noonan syndrome （NS） is a disorder characterized by dysmorphic facial features, short stature, hyperorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
Defects in RAF1 are the cause of LEOPARD syndrome type 2 （LEOPARD2） [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hyperorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
Similarity : Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD （Ras-binding） domain.
Database links : UniProtKB/Swiss-Prot: P04049.1
宿主：Human、Murine、Rat （Rattus）、Bovine、Dog （Canine）等物種
磷酸化原癌基因c-Raf抗體應(yīng)用范圍: WB, ELISA, IP, IHC （p）, IHC （f）, IF
緩沖液：0.01m PBS,pH值7.4和10毫克/毫升BSA
儲存液：0.1%（w/v）*                                                       

phospho-ROCK2（Thr249） 磷酸化Rho相關(guān)蛋白激酶2抗體Y55105 0.1ml
phospho-ROCK2（Tyr256） 磷酸化Rho相關(guān)蛋白激酶2抗體Y55106 0.1ml
RPLP0 酸性核糖體磷蛋白大亞基P0抗體Y55107 0.1ml
ROS/c ros 活性氧簇ROS抗體Y55108 0.1ml
ROR Gamma/RORC/NR1F3 孤兒核受體抗體Y55109 0.2ml
RPS6 S6核糖體蛋白抗體Y55110 0.1ml
Phospho-S6 Ribosomal Protein （Ser235+Ser236） 磷酸化S6核糖體蛋白抗體Y55111 0.1ml
Phospho-S6 Ribosomal Protein （Ser240/244） 磷酸化S6核糖體蛋白抗體Y55112 0.1ml
Rb/P105 RB 成視網(wǎng)膜細胞瘤基因抗體Y55113 0.1ml
phospho-Rb/p105-Rb（Ser780） 磷酸化成視網(wǎng)膜細胞瘤抑癌蛋白抗體Y55114 0.1ml
phospho-FXYD1 （Ser83） 磷酸化磷酸神經(jīng)膜抗體Y55115 0.1ml
RRAD RRAD抗體Y55116 0.2ml
phospho-FXYD1 （Ser88） 磷酸化磷酸神經(jīng)膜抗體Y55117 0.1ml
Rsk2/MAPKAP Kinase 1b 核糖體S6激酶RSK2抗體Y55118 0.2ml
Phospho-RSK2 （Ser227） 磷酸化核糖體S6激酶RSK2抗體Y55119 0.1ml
Phospho-RSK2（Tyr529） 磷酸化核糖體S6激酶RSK2抗體Y55120 0.1ml
RSK3/Ribosomal S6 kinase 3 核糖體蛋白S6激酶家族RSK3抗體Y55121 0.2ml
Phospho-RSK3 （Thr353/356） 磷酸化核糖體蛋白S6激酶家族RSK3抗體Y55122 0.1ml
Phospho-RSK3 （Thr356/Ser360） 磷酸化核糖體蛋白S6激酶家族RSK3抗體Y55123 0.1ml
RSV 呼吸道合胞病毒抗體Y55124 0.1ml
RXR Alpha/RXRA/Retinoid X receptor 核受體RXRα抗體Y55125 0.1ml
RXR gamma 維甲酸受體G抗體Y55126 0.2ml
RXR beta/RXRB 核受體RXRβ抗體Y55127 0.1ml
RUNX1/AML1 急性髓細胞白血病1蛋白抗體Y55128 0.1ml
phospho-RUNX1（Ser266） 磷酸化急性髓細胞白血病1蛋白抗體Y55129 0.1ml
Runx3 Runx3抗體Y55130 0.1ml
SDH/S6PDH 6-磷酸山梨醇脫氫酶抗體Y55131 0.2ml
SDH/Sorbitol Dehydrogenase 山梨醇脫氫酶抗體Y55132 0.1ml
SDHA 琥珀酸脫氫酶復(fù)合體亞基A抗體Y55133 0.2ml
SDHD 線粒體琥珀酸脫氫酶D抗體Y55134 0.2ml
S100B S100B蛋白抗體Y55135 0.1ml
S-100/S100A1 S100A1抗體Y55136 0.1ml
S100A13 S100A13抗體Y55137 0.1ml
S100A14 S100A14/15抗體Y55138 0.1ml
S100A4 S100A4抗體Y55139 0.1ml
S100A6BP/CACYBP 鈣周期素結(jié)合蛋白抗體Y55140 0.2ml
SAMDC S-腺苷蛋氨酸脫羧酶抗體Y55141 0.1ml
S100A8 S100鈣結(jié)合蛋白A8抗體Y55142 0.1ml
S100A6 S100鈣結(jié)合蛋白A6抗體Y55143 0.1ml
S100-A9/MRP14/CFAG/Calgranulin B S100A9抗體Y55144 0.1ml
S100A11 S100鈣結(jié)合蛋白A11抗體Y55145 0.1ml
Shrimp tropomyosin 南美白對蝦過敏原蛋白（蝦原肌球蛋白）抗體Y55146 0.1ml
S100P S100鈣結(jié)合蛋白P抗體Y55147 0.1ml
SYP p38/Synaptophysin/SAP-1 突觸素抗體/突觸相關(guān)蛋白-1Y55148 0.1ml
SARS putative orflab polyprotein 冠狀病毒抗體Y55149 0.2ml
SARS S-protein 抗冠狀病毒表面S蛋白抗體Y55150 0.1ml
SAHH S腺苷L-同型半胱氨酸水解酶Y55151 0.2ml
SATB1 核基質(zhì)結(jié)合區(qū)結(jié)合蛋白1抗體Y55152 0.1ml
Phospho-SATB1 （Ser47） 磷酸化核基質(zhì)結(jié)合區(qū)結(jié)合蛋白1抗體Y55153 0.1ml
Sca1/Ly6A/E T淋巴細胞激活蛋白抗體磷酸化原癌基因c-Raf抗體Y55154 0.2ml